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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Hyaluronan synthase 2 protein (70-359AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Biotin
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q92819
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
HAS2
- 抗体英文名:
HAS2 Antibody, Biotin conjugated
- 抗体名:
HAS2;Hyaluronan synthase 2;Hyaluronate synthase 2;Hyaluronic acid synthase 2;HA synthase 2
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix (By similarity).风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验特异性的选择主要需要考虑四个方面:蛋白特异性、种属特异性、实验方法特异性、标记物的特异性。 (1)蛋白特异性 针对需要检测的蛋白查找抗体,几个细节要区分,重组表达的蛋白和内源性蛋白的检测,对抗体的要求是不一样的,注意查看抗体说明书的检测说明。如果重组蛋白不是全长表达,则需要注意抗体的免疫原区域是否在重组蛋白区域内。内源性蛋白最好能清楚其剪切与修饰的方式,特殊表型的蛋白需要进行序列比对,并结合抗体免疫原序列,查看交叉反应的情况。磷酸化蛋白检测需要确定具体位点,不同位点的磷酸化意味
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
for each species of primary antibody used. The biotin molecule is small and can be easily conjugated to immunoglobulin by amino substitution at alkaline pH without the loss of immunoglobulin activity. These secondary antibodies are quite inexpensive, readily
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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