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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human (E3-independent) E2 ubiquitin-conjugating enzyme protein (10-277AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q9C0C9
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB; Recommended dilution: WB:1:500-1:2000
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
UBE2O
- 抗体英文名:
UBE2O Antibody
- 抗体名:
Ubiquitin-protein ligase O antibody
- 规格:
100μg/50μg/20μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
| 规格: | 20μg | 产品价格: | ¥440.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate \'Lys-63\'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853).风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods. Solutions
基因工程抗体(genetically engineered antibody)
蛋白质酶解、 折叠和糖基化等修饰过程。酵母的生长速度(1 ~3 小时/代)比动物细胞快(约1 天/代),营养条件比 大肠杆菌更简单。并且,其所表达的蛋白质大都以可溶性形式分泌到培养基中,有利于后期纯化。目 前应用最多的是酿酒酵母和毕赤酵母,后者具有乙醇氧化酶基因(AOXI),在甲醇的存在下能迅速启动 转录,所以更适合高密度培养。酵母具有与高等真核生物类似的O -糖基化和N -糖基化,但是酵母的 糖链成分主要是甘露糖,并且糖链过长,这种过度糖基化有可能会影响抗体的空间结构,进而影响抗体的活性。有报道
:分别是竞争性蛋白质结合分析法[3]、放射性免疫分析法[4]和薄层色谱法[5]。在80年代,放射性免疫分析法得到了广泛的应用和改进,由于这种方法采用放射性核素标记,应用范围受到一定限制,为了进一步提高检测的灵敏度和操作的安全性,进入90年代又陆续发明了各种酶标记法和化学发光法的竞争性ELISA检测试剂盒,这一类检测方法的根本原理都是基于抗体抗原的免疫反应,所有这些试剂盒中采用的抗体全部是兔抗血清或者是经过特异亲和提纯之后的抗cAMP或cGMP的兔多抗,毫无疑问,兔多抗在约四十年的cAMP,cGMP定量
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