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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human 39S ribosomal protein L53, mitochondrial protein (8-113AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q96EL3
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, IHC; Recommended dilution: IHC:1:20-1:200
- 浓度:
>95%,Protein G purified
- 靶点:
MRPL53
- 抗体英文名:
MRPL53 Antibody
- 抗体名:
MRPL53 antibody;39S ribosomal protein L53 antibody;mitochondrial antibody;L53mt antibody;MRP-L53 antibody;Mitochondrial large ribosomal subunit protein mL53 antibody
- 规格:
100μg/50μg/20μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
| 规格: | 20μg | 产品价格: | ¥440.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
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文献和实验Probing Functions of the Ribosomal Peptidyl Transferase Center by Nucleotide Analog Interference
nucleotide analogs into the peptidyl transferase center, the active site located on the interface side of the large ribosomal subunit. This method combined with standard tests of ribosomal functions broadens the biochemical repertoire to investigate
by the coupling between liquid chromatography and tandem MS. The presence or absence of a given ribosomal protein in the defective particle is determined by comparing the MS intensities of its identified tryptic peptides with that of the mature large subunit
) 2、Input 条带清晰,co-IP 条带很弱? 设X为诱饵蛋白,Y 为靶蛋白,有可能为 X 和 Y 的相互作用本身比较弱 X 蛋白并不是 Y 的唯一,只部分 Y 与 X 互作 可以尝试反向拉,比如用 Y 去拉 X,可能效果比较好 3、我的 p 53 蛋白正好和抗体重链分子量接近,要如何减少抗体干扰? 建议方案: IP/WB 一抗使用不同种属来源,选择与IP抗体种属无交叉反应的二抗(Cross Adsorbed secondary antibody) 特殊二抗:比如仅识别重链或者轻链的二抗,或者仅
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