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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Bryonia dioica Ribosome-inactivating protein bryodin I protein (24-247AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Bryonia dioica
- 保质期:
6个月
- 抗原来源:
Bryonia dioica
- 目录编号:
P33185
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
/
- 抗体英文名:
Ribosome-inactivating protein bryodin I Antibody
- 抗体名:
Ribosome-inactivating protein bryodin I antibody;EC 3.2.2.22 antibody;BD1 antibody;rRNA N-glycosidase antibody
- 规格:
100μg/50μg/20μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
| 规格: | 20μg | 产品价格: | ¥440.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Isolation and Purification of Ribosome-Inactivating Proteins
Ribosome-inactivating proteins (RIPs) are cytotoxic N -glycosidases identified in plants, fungi, and bacteria. RIPs inhibit protein synthesis by virtue of their enzymatic activity, selectively cleaving a specific adenine residue from a highly
for the generation of ITs, although several other single-chain ribosome-inactivating proteins (RIPs) or type I RIPs are also available (3 ). Type I RIPs are RNA N -glycosidases displaying potent inhibition of protein synthesis in cell-free assays, but they lack
) 2、Input 条带清晰,co-IP 条带很弱? 设X为诱饵蛋白,Y 为靶蛋白,有可能为 X 和 Y 的相互作用本身比较弱 X 蛋白并不是 Y 的唯一,只部分 Y 与 X 互作 可以尝试反向拉,比如用 Y 去拉 X,可能效果比较好 3、我的 p 53 蛋白正好和抗体重链分子量接近,要如何减少抗体干扰? 建议方案: IP/WB 一抗使用不同种属来源,选择与IP抗体种属无交叉反应的二抗(Cross Adsorbed secondary antibody) 特殊二抗:比如仅识别重链或者轻链的二抗,或者仅
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