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- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y(p)-I-V) derived from Human Ephrin-B2
- 亚型:
/
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
/
- 适应物种:
Human,Mouse
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P52799
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB;WB:1:500-1:1000
- 浓度:
>95%,Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- 靶点:
EFNB2
- 抗体英文名:
Phospho-EFNB2 (Tyr316) Antibody
- 抗体名:
EFNB2;EPLG5;HTKL;LERK5;Ephrin-B2;EPH-related receptor tyrosine kinase ligand 5;LERK-5;HTK ligand;HTK-L
- 规格:
100μl
保存缓冲液
/功能
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al.( 2006) Blood;107(6):2330-2338.
Noren NK, et al.( 2004) Proc Natl Acad Sci USA;101(15):5583-5588
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文献和实验-phosphoserine的抗体。 摘抄: Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner
单抗主要是鼠单抗、兔单抗,多抗主要是兔多抗、山羊多抗等,其他种属来源抗体较少。不建议纠结于单抗好还是多抗好,能做出实验的抗体就是好抗体。 在选择上一般建议实验种属和抗体种属亲缘性越远越好,不宜同源。抗体不同种属会要影响接下来二抗的选配。特别是在免疫荧光双标实验中,需要在区别于实验种属的基础上,区分开两个指标的抗体种属来源,以便于二抗区别识别。 3. 关于性价比的选择 抗体的品质高低最终还是需要通过实验结果来呈现的,但是在选择抗体之前我们并无法准确判断抗体的效果
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
(苯丙氨酸),Thr(苏氨酸)取代了GFP上的Ser65(丝氨酸),与GFP相比,具有更强更稳定的绿色荧光。CFP(Cyan Fluorescent Protein)与此类似,也是GFP第66位上的酪氨酸Tyr被色氨酸(Thr)所取代的结果,发蓝色荧光。由此可见,GFP标签与其它突变体GFP、YFP、EYFP、CFP的序列非常的类似,只有1-2个氨基酸残基的变化。EarthOx GFP标签抗体(#E022030)用于IF实验 EarthOx GFP标签抗体(#E022030)用于WB实验,1道为阴性
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