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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around aa.305~309(A-P-T-S-F) derived from Human Synaptophysin
- 亚型:
/
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
/
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P08247
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB;WB:1:500-1:1000
- 浓度:
>95%,Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- 靶点:
SYP
- 抗体英文名:
SYP Antibody
- 抗体名:
SYP;Synaptophysin;Major synaptic vesicle protein p38
- 规格:
100μl
保存缓冲液
/功能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity Characterization of a multidomain adaptor protein, p140Cap, as part of a pre-synaptic complex."Ito H., Atsuzawa K., Sudo K., Di Stefano P. J. Neurochem. 107:61-72(2008) "A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation."
Tarpey P.S., Smith R., Pleasance E., et al. Nat. Genet. 41:535-543(2009)"
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文献和实验Application of Real-Time STED Imaging to Synaptic Vesicle Motion
intensities (as usually higher intensities are needed for live imaging at adequate frequencies compared to fixed-cell imaging). In this chapter, we review recent progress made with stimulated emission depletion (STED) microscopy in imaging single synaptic
of synaptic vesicle proteins and the presynaptic active zone.
A small repository of synaptic protein information
5. Incubate overnight @ 4oC in primary antibody diluted in PBT. 6. Wash in 4-6 changes of PNT over a 2hr period @ RT. 7. Incubate 1-2h in secondary antibody @ RT on nutator. 8. Wash in 4-6 changes PBT (15' each). 9. Mount in 80% glycerol
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