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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human SMAP2
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
/
- 标记物:
/
- 适应物种:
Human,Mouse,Rat
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q8WU79
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB,IHC,IF
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
SMAP2
- 抗体英文名:
SMAP2 Antibody
- 抗体名:
SMAP2 antibody;SMAP1L antibody;Stromal membrane-associated protein 2 antibody;Stromal membrane-associated protein 1-like antibody
- 规格:
100μl
保存缓冲液
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles.功能
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文献和实验制备蛋白提取物1. Prepare extracts from cultured cells or tissues with our Trident Extraction Kits.The total number of cells per ml and the cell equivalent loaded per lane of gel should be optimized specifically for each protein and antibody.2. Determine
-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
染色体免疫共沉淀(Chromatin immunoprecipitation,ChIP)方法程序
),剪切后的DNA小片段与结合蛋白结合(Isolate the chromatin. Shear DNA along with bound proteins into small fragments.) 第三步:用特异性 抗体 与DAN结合蛋白结合,用沉淀法分离复合体。反向交联操作释放出DNA,并消化蛋白质(Bind antibodies specific to the DNA-binding protein to isolate the complex
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