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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from Human SH-PTP2 around the phosphorylation site of Y542.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
/
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse,Rat
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q06124
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA; WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:40000
- 浓度:
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点:
PTPN11
- 抗体英文名:
Phospho-PTPN11 (Y542) Antibody
- 抗体名:
Tyrosine-protein phosphatase non-receptor type 11 antibody
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.功能
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文献和实验11 Detection of TGF Type II Receptor Hotspot Mutations: The BAT-RII Assay
in the TGF-β pathway detected thus far is mutation of the type II receptor (RII) in colon cancers, particularly in tumors with defective mismatch repair or microsatellite instability (MSI) (3 –5 ). These RII mutations occur in a repetitive region of coding
Non-fluorescent protein/RNA double-labeling
antibody (Boerhinger Mannheim) conjugated to alkaline phosphatase (diluted 1:2000 in PBT) for 2 hours at room temperature. 12. Wash with PBT 4 times for 20 minutes each wash. Rinse with alkaline phosphatase staining buffer (100mM NaCl, 50 mM MgCl2, 100
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.
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