- 询价
- cusabio
- CN
- CSB-MA268113
- 2025年08月29日
- WB IF IP Recommended dilution: Western blotting: 1:1000~1:3000, Immunofluorescence: 1:100Immunoprecipitation: 1:200
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- 询价记录
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Shipped at 4°C Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
- 克隆性：
多克隆
- 应用范围：
WB IF IP Recommended dilution: Western blotting: 1:1000~1:3000, Immunofluorescence: 1:100Immunoprecipitation: 1:200
- 抗体英文名：
NFκB p65 Mouse Monoclonal Antibody
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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