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- 文献和实验
- 技术资料
- 形态:
liquid
- 保存条件:
Shipped at 4°C Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
- 克隆性:
多克隆
- 应用范围:
WB IHC IF Recommended dilution: Western blotting: 1:1000~1:3000
- 抗体英文名:
Histone H3 Mouse Monoclonal Antibody
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
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