- ￥5600
- GeneTex
- 美国
- GTX64171
- 2025年07月14日
- WB, IP, Dot, ELISA, Neutralizing/Inhibition
- Rabbit
- Streptococcus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
His6-tagged full-size recombinant SLO of S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Streptococcus
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX64171
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IP, Dot, ELISA, Neutralizing/Inhibition
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Streptolysin O
- 抗体英文名：
Streptolysin O antibody
- 抗体名：
Streptolysin O 抗体
- 规格：
100 μl
Western blot analysis of culture supernatant from GAS strain Sa and GCS strain H46A with the anti-SLO antibody. Lane 1; Medium only Lane 2*; Culture supernatant of GAS strain Sa Lane 3**; Culture supernatant of GCS strain H46A The antibody was used at 1/2,000 dilution * Most of SLO in culture medium of GAS strain Sa is proteolytically degraded, accounting for low activity level of SLO in the culture of this strain. Condensed sample is analyzed in Fig. 2. ** The two faint bands below SLO band are partially degraded SLO.
Western blot analysis of concentrated culture supernatant of GAS strain Sa with the anti-SLO antibody. Culture supernatant was approximately 1,000~2,000 fold concentrated from culture medium by ammonium fractionation and heat-precipitation. Lane 1; Concentrated culture of GSA strain Sa Lane 2; Concentrated medium only
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文献和实验Activation of Mast Cells by Streptolysin O and Lipopolysaccharide
This chapter provides protocols to measure the reversible permeabilization of mast cells by streptolysin O (SLO) and to follow SLO-induced activation of mast cells by monitoring degranulation, activation of mitogen-activated protein kinases
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
seven times, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods
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