- ￥5300
- GeneTex
- 美国
- GTX64150
- 2025年08月08日
- WB, ICC/IF, IP, ELISA
- Rabbit
- E. coli
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Highly purified full-length recombinant E. coli RecA protein
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
E. coli
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
E. coli
- 目录编号：
GTX64150
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IP, ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
RecA
- 抗体英文名：
RecA antibody
- 抗体名：
RecA 抗体
- 规格：
50 μg
Immunoprecipitation of RecA protein from crude extract of E. coli cells. Antibody 2 μg was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.
Western blot analysis of RecA protein in crude extract of E. coli Lane 1, Purified RecA 8 ng. Lane 2, Wild type. Lane 3, ⊿recA mutant. Lane 4, ⊿lexA mutant (SOS genes overexpressed). Antibody used at 1/3,000 dilution. Cell extracts, 2 μg
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- 作者
- 内容
- 询问日期
文献和实验是大肠杆菌中recA基因座的产物，具有双重功能，能激活蛋白酶并能改变单链DNA分子。蛋白酶-激活活性控制SOS 反应；核酸酶活性涉及重组修复途径。
Flexible Genetic Engineering Using RecA Protein
The analysis or manipulation of small segments of DNA in plasmids has long been routine, but standard methods fail or are very time consuming when applied to long segments or complex mixtures of DNA. At present, RecA protein-based techniques
BAC Modification Using a RecA Expressing Shuttle Vector System
Bacterial artificial chromosomes (BAC) are F-factor plasmid based vectors that are maintained as 1 or 2 copy plasmids in their bacterial host strain. BACs have the capacity for maintaining large, often more than 300 kb fragments of mammalian DNA
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