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- 详细信息
- 技术资料
- 免疫原:
Recombinant full-size S. pombe Rad21 protein (His-tagged) expressed in E. coli
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Schizosaccharomyces pombe
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Yeast
- 目录编号:
GTX64147
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Reacts with S. pombe Rad21 protein. Not tested with other species
- 抗体英文名:
Rad21 (S. pombe) antibody
- 抗体名:
Rad21 (S. pombe) 抗体
- 规格:
50 μl
Identification of Rad21 protein in crude extract of S. pombe with anti-Rad21 antiserum. WT cells and rad21-9xPK cells grown in YS5S to 1x107, collected, washed and broken by glass beads. Twenty ug proteins per lane were run on SDS-PAGE. First antibody at indicated dilutions in PBST plus 2.5% skim milk was incubated at 4ºC overnight. Second antibody at 1/10,000 of anti-rabbit HRP in PBST was incubated at room temperature for 1 h. Detected by ECL (X-ray film). Rad21 protein migrated at the position of ~110 kDa in SDS-PAGE although the molecular mass is 67.8 kDa. Multiple bands of Rad21 reflect multiple phosphorylation states.
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