- ￥5600
- GeneTex
- 美国
- GTX64098
- 2025年07月09日
- WB, IP, Dot, ELISA, Neutralizing/Inhibition
- Rabbit
- Bordetella pertussis
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Immunization was Initiated with toxoid and boosted with native toxin
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Bordetella pertussis
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX64098
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IP, Dot, ELISA, Neutralizing/Inhibition
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Bordetella pertussis Toxin
- 抗体英文名：
Bordetella pertussis Toxin antibody
- 抗体名：
Bordetella pertussis Toxin 抗体
- 规格：
100 μl
Titration of antibody reactivity of anti-Pertussis antiserum by direct ELISA Plate was coated with 100 μg of pertussis toxin per well and 100 μl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate.
Detection of perussis toxin in culture medium of Bordetera pertussis strain Tohama by Western blotting using anti-perussis toxin antibody. 1. Culture medium of Bordetera pertussis. SDS-PAGE, silver-stained. 2. Purified pertussis toxin (200 ng). SDS-PAGE, silver-stained. 3. Western blot of culture medium of Bordetera pertussis as in 1. 4. Western blot of purified pertussis toxin (10 ng) The toxin consists of five subunits as indicated by S1 to S5.
Titration of pertussis toxin by direct ELISA using anti-pertussis toxin antiserum ELISA plate is coated with indicated amounts of pertussis toxin per well. Antiserum was used at 1/12,500 dilution. ELISA was performed as in Fig.2. Dynamic range was 100 pg to 10 ng under these conditions.
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文献和实验百日咳杆菌（ bordetella pertussis ）是人类百日咳的病原菌。百日咳杆菌为卵圆形短小杆菌，大小为 0.5 ～ 1.5 × 0.2 ～ 0.5um ，属鲍特氏菌属 bordetella) ，无鞭毛、芽胞。革兰氏染色阴性。用甲苯胺蓝染色可见两极异染颗粒。专性需氧，初次分离培养时营养要求较高，需用马铃薯血液甘油琼脂培养基（即鲍～金氏培养基）才能生长。经 37 ℃ 2 ～ 3 天培养后，可见细小、圆形、光滑、凸起、银灰色、不透明的菌落，周围有模糊的溶血
Preparation of Cytotoxic Antibody-Toxin Conjugates
) that inactivates eukaryotic ribosomes by a specific irreversible covalent modification of the ribosomal RNA (2 ). The B chain binds to cell surface galactose-containing oligosaccharide residues. Following receptor-mediated endocytosis of toxin bound to the cell
Bordetella pertussis , the causative agent of whooping cough, secretes a plethora of virulence factors contributing to the onset and characteristic of the disease. The exotoxin pertussis toxin (PT) is regarded as particularly important
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