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中文详细描述：
HT Nucleofector高通量细胞核转染平台使用384孔电转板，适合于高通量的筛选实验。在具备Nucleofector高效转染、DNA直接入核、无需自行摸索复杂的电转条件等优势外，还具备快速转染（1分钟/384孔）、每块板可最多设置384种转染条件、可与自动化工作站进行整合等特点，是高通量筛选实验的理想选择。
英文详细描述：
HT Nucleofector™ System
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HT Nucleofector™ Device
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384-well Nucleocuvette™ Plates
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HT Nucleofector™ Device
The High-Throughput Nucleofection™ Platform
The new HT Nucleofector™ System is an independent platform for highthroughput Nucleofection™ in 384-well format. With an extremely fast plate processing time of one minute it is perfectly suited for screening applications with maximum reproducibility. Furthermore cell storage time in Nucleofector™ Solution is reduced to a minimum and all existing 96-well Shuttle™ Protocols can be used without further optimization.
Benefits
The HT Nucleofector™ System consists of
Technical SpecificationsDimensions (w×d×h) 384-well Nucleofector™ Plate Handler:
40 cm × 42 cm × 15 cm (15.7 × 16.5 × 5.9 in)
384-well Nucleofector™ Power Supply:
13.5 cm × 50 cm × 45 cm (5.3 × 19.6 × 17.7 in)Weight 384-well Nucleofector™ Plate Handler:
10 kg (22.04 lb)
384-well Nucleofector™ Power Supply:
14 kg (30.86 lb)
Ordering InformationPlease contact Customer Service for pricing information.
AAU-1001 384-well Nucleofector™ System
Includes power supply, plate handler, laptop, and softwareAWT-1001 384-well Nucleofector™ Installation and Training AWU-1001 384-well Nucleofector™ Service Contract*
Can be purchased at any time during the guarantee period
*Comprises diagnosis and repair of the device along with all replacement parts.-
Plate Handler Unit - The carousel of this unit offers two positions for 384-well Nucleocuvette™ plates, thus allowing loading and processing of two plates in parallel
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Power Supply – Assembled in a 19" format the power supply can be positioned horizontally or vertically
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HT Nucleofector™ Operation Software - The unique and intuitive software allows easy parameterization of high throughput experiments. The software manages up to 384 different programs per plate, thus each well can be adressed individually
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Fast - Processes a 384-well plate in one minute, carousel capable of handling two plates
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High performance combined with minimum material consumption - Nucleofection™ of low cell numbers down to 2x104 cells
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Easy-to-use - uses existing 96-well Shuttle™ Protocols
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Automation compatible - seamless integration into automated liquid handling environments
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文献和实验相关实验
小鼠5羟色胺(5-HT) 酶联免疫 分析（ELISA） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 5羟色胺(5-HT)的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 5羟色胺 (5-HT) 水平。用纯化的 小鼠 5羟色胺 (5-HT) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 5羟色胺 (5-HT) ， 再与HRP 标记
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
assays for measuring the binding of radioligands to two major types of receptors for 5?hydroxytryptamine (5?HT or serotonin), 5?HT1 and 5?HT2 receptors, in homogenates of brain tissue or cloned into cells in culture. The specific receptor subtypes covered
做好准备工作之后，先把旧的培养基倒掉。再用PBS洗两遍，然后加0.05%胰酶/EDTA，剂量根据培养瓶大小定，一般是25cm 2 的瓶子大约0.8-1ml，轻摇10——15秒，使消化液均匀覆盖瓶底即可，再倒掉。置培养箱3-5分钟(看消化效果而定)，等大部分细胞呈流沙状滑落时，即加入培养基终止消化，一般以1:5左右的比例传代(视计数结果和看细胞长满的程度而定)。这种方法养比较顽强的细胞很好，既节省步骤，也降低了离心和较长时间消化对细胞带来的损伤，其实大家也可以试试用这种方法养别的细胞。主要做
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HT Nucleofector高通量细胞核转染平台
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