- ¥578
- GIBCO
- 进口
- 27016021
- 2025年07月13日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
RPMI 1640 Medium, no folic acid
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
描述
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. We offer a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Folic acid |
The complete formulation is available.
Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
规格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Liquid |
| HEPES Buffer: | No HEPES |
| Serum Supplementation: | Standard Serum Supplementation |
| Sodium Pyruvate Additive: | No Sodium Pyruvate |
| Other Additives: | No Folate (No Folic Acid) |
| Product Line: | Gibco® |
| Product Size: | 500 mL |
| Volume (Metric): | 500 ml |
| Green Features: | Fewer resources used, Less waste |
| Shipping Condition: | Room Temperature |
内容及储存
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关专题 在实验中我们经常会需要自己配置RPMI1640培养基，在配置的过程中需要注意哪些？哪些因素会影响到RPMI1640培养基的配置效果呢?生物帮为您盘点 了配制RPMI1640培养基13点需知，详情请看下文： 1)配制培养基最好使用新制备的三蒸水。一般在试验前当天或前一天制备为好。调节pH值的酸碱溶液也应该使用这种水配制。 2)培养液配好后，应先抽取少许放入培养瓶内，于37℃温箱内置24～48hr
phoolishkate：脂质体转染后必须用不含抗生素的培养基吗？我的培养基都加了青链霉素，转染后是不是不能用？丁香园网友applezph认为：由于脂质体转染对细胞存在一定毒性，一般说来转染前的一次传代就应用不含抗生素的培养基，转染后的话我觉得也不应该加青链（我都是这么做的）当然如果对自己的操作实在没有把握的话，我认为你可以考虑试试转染后加，但不要太早，4-6小时换液用无抗生素的，可以24后再换用含抗生素培养基丁香园网友qxlbljys认为：1.做细胞转染时，由于转染介质如脂质体可增加膜的通透
40 维生素 B 12 0.005 RPMI-1640(A) 无酚红细胞培养基（粉末型）成分
技术资料暂无技术资料 索取技术资料
