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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
EX-CELL® 293 Serum-Free Medium for HEK 293 Cells
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
Application
EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.
法律信息
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Protocols & Applications
ECACC Handbook-Fundamental Techniques in Cell Culture
性质
Related Categories Additional Vaccine Media, Gene Therapy, Vaccine Media, 无血清培养基, 疫苗生产,
更多...
Quality Level GMP
description for research or for further manufacturing use
form liquid
suitability suitable for cell culture
storage temp. 2-8°C
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文献和实验1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
Generating stable cell lines in HEK293
Generating stable cell lines in HEK293 Prior to transfection, it is recommended that you linearize your pcDNA gene construct. Linearizing will decrease the likelihood of the vector integrating into the genome in a way
细胞名称 293 和 293T种属人组织来源 293细胞是转染腺病毒E1A基因的人肾上皮细胞系，293T细胞由293细胞派生，同时表达SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。用磷酸钙转染效率可高达50%。蛋白表达水平高，转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬时转染293T细胞是过表达蛋白并获得细胞内及细胞外（分泌的或膜的）蛋白的便捷方式。形态特点 上皮细胞致瘤性 +产物或抗原 大T抗原染色体核型 亚三倍体人细胞系。染色体众数为64，30%的细胞
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