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文献和实验Translation Using a Wheat-Germ Extract
Wheat-germ extract provides a reliable, convenient, and easy-to-use system to initiate translation from a wide variety of viral, prokaryotic, and eukaryotic mRNAs and produce full-length polypeptide products (1 ). Translation reactions in vitro
Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
of properly folded, eukaryotic proteins remains a challenging task. Current wheat-germ-based cell-free in vitro translation systems present a plausible alternative for protein synthesis since majority of eukaryotic proteins could be obtained in their native
In Vitro Translation of mRNA in a Wheat Germ Extract Cell-Free System
The wheat germ extract in vitro translation system has been used widely for faithful and efficient translation of viral and eukaryotic messenger RNAs in a heterologous cell-free system (1 –9 ). With respect to the yield of translation products
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