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- Bioss
- bs-0295G-SA
- 2026年10月10日
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100ul/1ml
| 规格： | 100ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥3600.0 |
| 产品编号 | bs-0295G-SA |
| 英文名称 | Goat Anti-Rabbit IgG H&L, SA conjugated |
| 中文名称 | 链霉亲和素标记山羊抗兔IgG H&L |
| 英文别名 | Rabbit Immunoglobulin G; Rb IgG; |
| 交叉反应 | Rabbit |
| 抗体来源 | Goat |
| 免疫原 | Native Rabbit IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
Dual- and Triple-Color Fluorospot
, in PBS containing 0.1% bovine serum albumin (BSA) (seeNote 8). Add 100 μl/well and leave the plate for 2 h at room temperature. c. Wash the plate as in step 6 above. d. Dilute secondary detection reagents, i.e., mAb anti-FITC
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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