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1mg/10mg
| 规格: | 1mg | 产品价格: | ¥1280.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥6400.0 |
| 产品编号 | bs-0327R |
| 英文名称 | Rabbit Anti-Bovine IgM |
| 中文名称 | 兔抗牛IgM |
| 英文别名 | Immunoglobulin M |
| 产品应用 | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:200-1000, IF, ELISA=1:2000-10000 Not yet tested in other applications. |
| 交叉反应 | Bovine |
| 抗体来源 | Rabbit |
| 免疫原 | Native Bovine IgM |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:200-1000} |
| {ELISA} | {1:2000-10000} |
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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