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- Bioss
- bs-0326G
- 2025年10月10日
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产品信息以Bioss网站为准
- 规格：
1mg/10mg
| 规格： | 1mg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥6400.0 |
| 产品编号 | bs-0326G |
| 英文名称 | Goat Anti-Bovine IgG H&L |
| 中文名称 | 羊抗牛IgG H&L |
| 英文别名 | Bovine Immunoglobulin G; Bov IgG; |
| 产品应用 | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:200-1000, IF, ELISA=1:2000-10000 Not yet tested in other applications. |
| 交叉反应 | Bovine |
| 抗体来源 | Goat |
| 免疫原 | Native Bovine IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:200-1000} |
| {ELISA} | {1:2000-10000} |
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文献和实验[IF={{ 5.401 }}] {Xia, Miaomiao, et al. "Protein self-assembly via Zr 4+ ions on spore-based microspheres for immunoassays." Sensors and Actuators B: Chemical(2017).} {Other} {Bovine}
E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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