- ￥1280 - 6400
- Bioss
- bs-0294M
- 2025年10月10日
- 产品信息以Bioss网站为准
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￥480
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- 规格：
1mg/10mg
| 规格： | 1mg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥6400.0 |
| 产品编号 | bs-0294M |
| 英文名称 | Mouse Anti-Goat IgG H&L |
| 中文名称 | 小鼠抗羊IgG H&L多克隆抗体 |
| 英文别名 | Goat Immunoglobulin G |
| 交叉反应 | Goat |
| 抗体来源 | Mouse |
| 免疫原 | Native Goat IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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文献和实验[IF={{ 3.481 }}] {Jinzhu Han. et al. Circ_0027599 elevates RUNX1 expression via sponging miR-21-5p on gastric cancer progression. 2021 May 25} {WB} {Human}
[IF={{ 2.78 }}] {Liu et al. Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. (2018) Int.J.Mol.Med. 41:2609-2618} {IF} {Goat}
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
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