- ￥480 - 3600
- Bioss
- bs-0311R-PE
- 2025年10月10日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥3600.0 |
| 产品编号 | bs-0311R-PE |
| 英文名称 | Rabbit Anti-Biotin, PE conjugated |
| 中文名称 | PE标记的兔抗生物素抗体IgG H&L |
| 英文别名 | Rabbit Anti-Biotin IgG H&L (PE) |
| 产品应用 | IF=1:100-1000, Flow-Cyt=1:100-1000, ICC/IF=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Biotin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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