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- Bioss
- bs-0295G-Cy5.5
- 2026年10月10日
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100ul/1ml
| 规格： | 100ul | 产品价格： | ￥360.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥2400.0 |
| 产品编号 | bs-0295G-Cy5.5 |
| 英文名称 | Goat Anti-Rabbit IgG H&L, Cy5.5 conjugated |
| 中文名称 | Cy5.5标记的羊抗兔IgG H&L |
| 英文别名 | Goat Anti-Rabbit IgG H&L (Cy5.5); Immunoglobulin G; |
| 产品应用 | IF=1:100-1000, ICC/IF=1:100-1000, Flow-Cyt=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Rabbit |
| 抗体来源 | Goat |
| 免疫原 | Native rabbit IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
Dual- and Triple-Color Fluorospot
, in PBS containing 0.1% bovine serum albumin (BSA) (seeNote 8). Add 100 μl/well and leave the plate for 2 h at room temperature. c. Wash the plate as in step 6 above. d. Dilute secondary detection reagents, i.e., mAb anti-FITC
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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