Rabbit anti-Human IgM pAb, AP conj.(bs-0345R-AP)-100ul/1ml产品图

Rabbit anti-Human IgM pAb, AP

conj.(bs-0345R-AP)-100ul/1ml
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  • ¥640 - 4800
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  • bs-0345R-AP
  • 2026年10月10日
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    • 文献和实验
    • 技术资料
    • 规格:

      100ul/1ml

    规格:100ul产品价格:¥640.0
    规格:1ml产品价格:¥4800.0
    产品编号bs-0345R-AP
    英文名称Rabbit Anti-Human IgM, AP conjugated
    中文名称碱性磷酸酶(AP)标记的兔抗人IgM
    英文别名Mouse Anti-Human IgM (AP); Immunoglobulin M;
    中文别名兔抗人-IgM;
    产品应用WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原Native Human IgM
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1.0 mg/ml
    储存液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
    保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.

     

    应用推荐稀释比例
    {WB}{1:1000-10000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-1000}
    {ELISA}{1:1000-10000}

     

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    图标文献和实验
    相关实验
    • WB操作规程

      、HCMV项目测血时,血清稀释度为1:500-1:1000。 4. KSHV、MCMV、HCMV项目WB鉴定时,一抗如为IgG型,二抗采用AP-Anti mouse IgG(Whole molecule),SIGMA,CAT#A-3562; 一抗如为IgM型,二抗采用AP-Anti mouse IgGAM,ZYMED,CAT#62-6422。 5.多肽项目WB鉴定,二抗全部采用AP-Anti mouse IgG(Whole molecule),SIGMA,CAT

    • Studies of the Ubiquitin Proteasome System

      with Nonradioactive Tag (Biotin) Alternate Protocol 10: Generation of Tagged Ubiquitin Expression Plasmids Basic Protocol 18: Immunoblotting with Anti‐Ubiquitin Generation and Purification of Rabbit Anti‐Ubiquitin Antibodies Basic Protocol 19: Generation

    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

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