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- 2025年10月17日
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1KU/2KU
| 规格： | 1KU | 产品价格： | ￥700.0 |
|---|---|---|---|
| 规格： | 2KU | 产品价格： | ￥1100.0 |
| 产品编号 | bs-0220P |
| 英文名称 | Alkaline Phosphatase(≥5,500 U/mg) |
| 中文名称 | 碱性磷酸酶 (进口分装 ≥5,500 U/mg)蛋白 |
| 英文别名 | Alkaline Phosphatase from calf intestine; Phosphatase, Alkaline from bovine intestinal mucosa; Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); ALP-1; ALP; ALPP; ALPPL2; ALPPL; PALP; PLAP-1; PLAP; |
| 理论分子量 | ~160 |
| 保存条件 | Stored at 2-8℃. Do not freeze. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Alkaline phosphatase is highly purified from calf intestine. Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays. |
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文献和实验[IF={{ 4.9 }}] {Yao Yu. et al. Dual-mode colorimetric–fluorometric immunoassay for foodborne parasitic infection. MICROCHEM J. 2024 Dec;207:111778} {} {}
[IF={{ 3.864 }}] {Xie W et al. A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.Analyst. 2018 Jun 11;143(12):2901-2907.} {Other} {}
) 20ml 100ml ) 500ml Distilled water 55ml 275ml ) b. Naphthol ASBI stock Naphthol ASBI phosphate 25mg N:N Dimethyl formamide 10ml Distilled water 10ml Adjust to pH 8.0 with 1M sodium carbonate if necessary then add
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
ALKALINE PHOSPHATASE SECTIONS Fresh Frozen (jejunal Biopsy) or cold Formal calcium/Gum Sucrose fixed Cryostat 5-7micron thick. SOLUTIONS a. 0.2M Tris HCL buffer pH
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