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- 规格:
1mg
| 产品编号 | bs-23299PB |
| 英文名称 | HCY/BSA |
| 中文名称 | 同型半胱氨酸/高胱氨酸/血同 |
| 英文别名 | homocystine; D,L-homocysteinethiolactone HCl; DL-Homocysteine thiolactone hydrochloride; DL-Homocysteine Thiolactone Hydrocloride; 3-aminodihydrothiophen-2(3H)-one hydrochloride (1:1); (3R)-3-aminodihydrothiophen-2(3H)-one hydrochloride (1:1). |
| 中文别名 | 高胱氨酸牛血清白蛋白偶联物; |
| 性状 | Liquid |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group. Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine. |
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文献和实验Maxisorb 442404)Micro test tubes, 大头针或喷火枪药品试剂:(a) Buffer A-0(b) BSA标准品 (Bio-Rad, bovine serum albumin, 100 μg/mL)(c) 未知浓度样本 (unknown BSA, 0.8 mL, 由教师调配发给)(d) Dye Reagent (Bio-Rad 500-0006) 先以水稀释四倍备用。 以染料Coomassie brilliant blue G-250 配制的溶液,勿与胶体电泳染色用的CBR-250
除>95%的蛋白提取和样品制备过程中所使用的去垢剂,而蛋白和多肽损失极少。此树脂适用于常用去垢剂的去除,如浓度在0.5-1%的SDS、Triton X-100、NP-40和CHAPS。目前有预装的离心柱和离心板形式,最多可处理100 µL样品。另外,您也可以购买HiPPR Detergent Removal Spin Column Kit,其中包含树脂浆和空的离心柱,便于您自己调整样品体积。 整个处理过程相当简单,仅需15分钟。首先是离心去除保存液,之后用缓冲液平衡树脂并离心,重复
Bromodeoxyuridine Staining of Mammary Tissue
oC in a humid box. 3. Rinse 2 x 3 min in PBS. 4. Treat sections with 0.3% hydrogen peroxide for 10 minutes at room temperature. 5. Rinse 2 x 3 min in PBS. 6. Treat with 2N HCl for 1 hour at 37o C. 7. Rinse 2 x 5 min in 0.1M borate buffer pH
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