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- bs-0330Rs
- 2025年10月10日
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| 产品编号 | bs-0330Rs |
| 英文名称 | Rabbit Anti-Mouse Kappa Chain whole serum |
| 中文名称 | 兔抗小鼠k-链抗血清 |
| 英文别名 | Mouse Ig kappa chain C region; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; kappa light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; IGKC_MOUSE. |
| 交叉反应 | Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from Mouse Kappa light chain |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | Unpurified |
| 克隆类型 | Polyclonal |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. |
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文献和实验. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200 in TBS-BSA NaN 3 . 4- Wash thrice in TBS 0.05M pH7.5 + 0.01% Tween 20.
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