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- 应用范围:
产品信息以Bioss网站为准
- 规格:
1ml
| 产品编号 | bs-0309Rs |
| 英文名称 | Rabbit Anti-Pig IgG H&L whole serum |
| 中文名称 | 兔抗猪IgG H&L抗血清 |
| 英文别名 | Pig Immunoglobulin G |
| 交叉反应 | Pig |
| 抗体来源 | Rabbit |
| 免疫原 | Native Pig IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | Unpurified |
| 克隆类型 | Polyclonal |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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文献和实验[IF={{ 5.4 }}] {Dedong Wang. et al. Ubiquitination-dependent degradation of nucleolin mediated by porcine circovirus type 3 capsid protein. J VIROL. 2023 Nov 30} {Other} {}
[IF={{ 4 }}] {Dedong Wang. et al. E3 ligase RNF2 inhibits porcine circovirus type 3 replication by targeting its capsid protein for ubiquitination-dependent degradation. J VIROL. 2024 七月 24} {WB} {Pig}
[IF={{ 2.4 }}] {Jie Zhao. et al. Ubiquitination-dependent degradation of DHX36 mediated by porcine circovirus type 3 capsid protein. VIROLOGY. 2025 Jan;:110419} {} {}
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody
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