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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rice Helix-loop-helix DNA-binding domain containing protein, expressed (LOC_Os11g32100)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg/20μg
| 规格: | 100μg | 产品价格: | ¥8520.0 |
|---|---|---|---|
| 规格: | 20μg | 产品价格: | ¥5268.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
LOC_Os11g32100Uniprot No.:
Q2R3F6别名:
/种属:
Oryza sativa subsp. japonica (Rice)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
69.5 kDa表达区域:
1-524aa氨基酸序列:
MLPRFHGAMWMQDDGGGDQEHGQAAPPGQEQHHHDQHLMALAAAAAGGAGFGAAQAPAPLLDEDWYFDAAGGGGGGAHGSMMLGLSSVHGGIGAGTSGGGHGQQFSLLNMGAAAAPFDVSGFDLGIACGGVGGGGDVVSFLGGGNASNTALLPVGNAGFLGTFGGFGTAASQMPEFGGLAGFDMFDAGAVNTGG_S_SSSSSAAAAAASASAHVSNTAPFSGRGKAAVLRPLDIVPPVGAQPTLFQKRALRRNAGEDDDDKKRKAAAGAGAGALSADGADMVLDDGDDDGLSIDASGGLNYDSEDARGGEDSGAKKESNANSTVTGDGKGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIEYLKELLQKINDLQNELESSPATSSLPPTPTSFHPLTPTLPTLPSRIKEEICPSALPSPTGQQPRVEVRLREGRAVNIHMFCARRPGLLLSAMRAVEGLGLDVQQAVISCFNGFTLDIFKAEQCKDGPGLLPEEIKAVLMQSAGFHTMI蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"The Rice Annotation Project Database (RAP-DB): hub for Oryza sativa ssp. japonica genome information." Ohyanagi H., Tanaka T., Sakai H., Shigemoto Y., Yamaguchi K., Habara T., Fujii Y., Antonio B.A., Nagamura Y., Imanishi T., Ikeo K., Itoh T., Gojobori T., Sasaki T. Nucleic Acids Res. 34:D741-D744(2006)Function:
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文献和实验α螺旋-转角-α螺旋基元(helix-turn-helix motif)
这是最早在原核基因的激活蛋白和阻遏蛋白中发现的调控蛋白, 是一种同型二聚体。构成同型二聚体的每个单体由20个氨基酸残基的小肽组成α螺旋-转角-α螺旋结构, 其中一个螺旋为识别螺旋(recognition helix), 负责识别DNA大沟的特异碱基序列。另一个螺旋没有碱基特异性, 与DNA磷酸戊糖链骨架接触。在与DNA特异结合时, 以二聚体形式发挥作用, 结合靠蛋白质的氨基酸侧链与特异碱基对之间形成氢键。
Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides
The design of molecules that recognize specific sequence on the deoxyribonucleic acid (DNA) double helix would provide interesting tools to interfere with DNA information processing at an early stage of gene expression. This chapter describes
Targeted Mutagenesis Mediated by the Triple Helix Formation
Oligonucleotides can bind in the major groove of duplex DNA and form triple helices in a sequence-specific manner 1 –4 ). Progress in elucidating the third strand binding code has raised the possibility of developing nucleic acids as sequence
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