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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-16115R |
| 英文名称 | Cadherin28 Rabbit pAb |
| 中文名称 | 钙粘蛋白28抗体 |
| 英文别名 | FLJ23834; Cadherin28; Cadherin-like protein 28; Cadherin-related family member 3; Cdhr3; CDHR3_HUMAN; FLJ43271; Hypothetical protein FLJ23834; Hypothetical protein LOC222256; MGC133292; MGC133293. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cadherin28 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 96 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Cell adhesion > Cadherins Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Cadherins Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Binding Proteins |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein (By similarity). |
| 相似性 | Contains 6 cadherin domains. |
| 功能 | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

U251(Human) Cell Lysate at 30 ug
Primary: Anti-Cadherin28 (bs-16115R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 96 kD
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文献和实验Cadherin-Mediated Cell-Cell Interactions
P-Cadherin Human, mouse, bovine Placenta (mouse), skin, breast (myoepithelial cells), prostate (basal cells) R-Cadherin (cadherin
家兔(Rabbit,Oryctolagus curiculus)
家兔(Rabbit,Oryctolagus curiculus) 一、生物学特性和解剖生理特点 1.家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。 2.家兔体小力弱、胆小怕惊、怕热、怕潮,喜欢安静、清洁、干燥、凉爽的环境,不能忍受污秽的条件。 3.是食草类(Herbivorous)单胃动物。饲养原则是以青粗食米为主,精饲料为辅。喜欢独居,白天活动少,都处于假眠或休息状态,夜间活动大,吃食多。有啃木、扒土的习惯
实验28 真核生物钙调素的酶联免疫测定法 原理 本法是一种测定抗体的竞争性固相酶联免疫测定法(enzyme linked immunosorbent assay,ELISA)。先将抗原──CaM与固相载体(聚苯乙烯微量滴定板)结合,然后将经待检CaM(标准样品或检样)部分中和的兔抗CaM抗体加入微量滴定板孔中,抑制固相CaM和抗体的反应。接着,废弃上清液,洗涤固相表面,加入辣根过氧化物酶标记的葡萄
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