phospho-HDAC4 (Ser246) Rabbit pAb(bs-10325R)-50ul/100ul/200ul
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phospho-HDAC4 (Ser246) Rabbit

pAb(bs-10325R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-10325R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-10325R
    英文名称phospho-HDAC4 (Ser246) Rabbit pAb
    中文名称磷酸化组蛋白去乙酰化酶4(Ser246)抗体
    英文别名HDAC4(phospho-Ser246); HDAC4(phospho-S246); p-HDAC4(Ser246); p-HDAC4(S246); HD 4; HD4; HDAC 4; HDAC A; HDACA; Histone Deacetylase 4; Histone Deacetylase A; KIAA0288; EC 3.5.1.98; HA6116; HDA4_CAEEL; Histone deacetylase 4; CeHDA-7; Histone deacetylase 7.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human (Mouse, Rat, Chicken, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthesised phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量119 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Heart > Hypertrophy

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Acetylation

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Acetylation > HDACs > Class II / Hda1 Class

    Stem Cells > Signaling Pathways > Wnt > HDACs

    亚基Interacts with HDAC7. Homodimer. Homodimerization via its N-terminal domain. Interacts with MEF2C, AHRR, and NR2C1. Interacts with a 14-3-3 chaperone protein in a phosphorylation dependent manner. Interacts with BTBD14B. Interacts with KDM5B. Interacts with MYOCD. Interacts with MORC2. Interacts with ANKRA2.
    亚细胞定位Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.
    组织特异性Ubiquitous.
    翻译后修饰Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350 impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
    相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
    功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    phospho-HDAC4 (Ser246) Rabbit
    P‌‌araformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-HDAC4 (Ser246)) Polyclonal Antibody, Unconjugated (bs-10325R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    phospho-HDAC4 (Ser246) Rabbit
    Sample:
    Hela(Human) Cell Lysate at 30 ug
    293T(Human) Cell Lysate at 30 ug
    Primary: Anti- Phospho-HDAC4 (Ser246) (bs-10325R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 119 kD
    Observed band size: 119 kD

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    该产品被引用文献

    [IF={{ 8.2 }}] {Zhu Xue-Xue. et al. Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling. CELL COMMUN SIGNAL. 2024 Dec;22(1):1-23} {WB} {Rat}

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    phospho-HDAC4 (Ser246) Rabbit pAb(bs-10325R)-50ul/100ul/200ul
    ￥1180 - 2800