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- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13245R |
| 英文名称 | G gamma4 Rabbit pAb |
| 中文名称 | G蛋白偶联受体γ4抗体 |
| 英文别名 | GNG4; GNGT4; Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-4 subunit; GBG4_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Dog, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human G gamma4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 8 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins |
| 亚基 | G proteins are composed of 3 units, alpha, beta and gamma. Interacts with beta-1 and beta-2, but not with beta-3. |
| 亚细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). |
| 组织特异性 | Brain, kidney, pancreas, skeletal muscle and faintly in cardiac muscle. |
| 相似性 | Belongs to the G protein gamma family. |
| 功能 | Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G gamma4 interacts with beta-1 and beta-2, but not with beta-3. It is expressed in brain, kidney, pancreas, skeletal muscle and faintly in cardiac muscle. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 5.5 }}] {Liang L et al. Distinguishable Prognostic Signatures of Left-and Right-Sided Colon Cancer: a Study Based on Sequencing Data.Cell Physiol Biochem. 2018;48(2):475-490.} {IHC} {Human}
[IF={{ 4.375 }}] {Liang Liang. et al. GNG4 Promotes Tumor Progression in Colorectal Cancer. J Oncol. 2021;2021:9931984} {IHC} {Human}
[IF={{ 15.7 }}] {Wu Fangfang. et al. A visual cortical-lateral posterior thalamic nucleus circuit regulates depressive-like behaviors in male mice. NAT COMMUN. 2025 Feb;16(1):1-17} {WB,IHC} {Mouse}
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
for most in vitro studies of alveolar epithelial function (e.g., transport and various metabolic pathways). The primary culture of human alveolar epithelial cells involves isolation, purification, and culture of alveolar epithelial type II (ATII) cells from human
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
, Algermissen, B. et al. 3. Purification of rabbit liver aldehyde oxidase by affinity chromatography on benzamidine Sepharose 6B. J. Chromatogr. (1989), 475 363-72, Stell, J. G. P. et al. 4. The Crystal Structure of a T Cell Receptor in Complex
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