Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-CNKSR2/KSR2 (bs-8029R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD
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- 详细信息
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-8029R |
| 英文名称 | CNKSR2/KSR2 Rabbit pAb |
| 中文名称 | Ras激酶抑制因子连接增强蛋白2抗体 |
| 英文别名 | CNKR 2; CNK2; CNKSR2; Connector enhancer of kinase suppressor of Ras 2; Connector enhancer of KSR2; KIAA0902; KSR2; RP11 450P7.2; CNKR2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CNKSR2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 114 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Adapters > Cytoplasmic Signal Transduction > Adapters > Transmembrane Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Ras Family |
| 亚基 | Interacts with RAF1, RAB2L and RAL GTPase proteins. |
| 亚细胞定位 | Cytoplasm. Membrane; Peripheral membrane protein. |
| 翻译后修饰 | Phosphorylated on tyrosine. |
| 相似性 | Belongs to the CNKSR family. Contains 1 CRIC domain. Contains 1 DUF1170 domain. Contains 1 PDZ (DHR) domain. Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. |
| 功能 | May function as an adapter protein or regulator of Ras signaling pathways. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CNKR2 may function as an adapter protein or regulator of Ras signaling pathways. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (bs-8029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (bs-8029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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CNKSR2/KSR2 Rabbit pAb(bs-8029R)-50ul/100ul/200ul
¥1180 - 2800








