- ￥1180 - 2800
- Bioss
- bs-7731R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7731R |
| 英文名称 | TRIP1 Rabbit pAb |
| 中文名称 | 甲状腺受体相互作用蛋白1抗体 |
| 英文别名 | 26S protease regulatory subunit 8; Cim3; MSUG1 protein; p45 antibody p45/SUG; Proteasome(prosome macropain) 26S subunit ATPase 5; Proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; Proteasome prosome macropain 26S subunit ATPase 5; Proteasome subunit p45; PSMC 5; PSMC5; Rpt6; S8 antibody SUG 1; SUG1; Tat binding protein homolog 10; TBP 10; TBP10; Thyroid hormone receptor interacting protein 1; Thyroid receptor interactor 1; TRIP 1; TRIP1; TRIP1(SUG1). |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TRIP1/PSMC5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Proteasome |
| 亚基 | Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR) (By similarity). Interacts with NDC80. Interacts with PAAF1. |
| 亚细胞定位 | Cytoplasm (Potential). Nucleus (Potential). |
| 相似性 | Belongs to the AAA ATPase family. |
| 功能 | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Human SUG1 (also known as p45, Rpt6, Cim3 and PSMC5) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquitinated proteins in an ATP dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIP1) Polyclonal Antibody, Unconjugated (bs-7731R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIP1) Polyclonal Antibody, Unconjugated (bs-7731R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- TRIP1 (bs-7731R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- TRIP1 (bs-7731R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
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TRIP1 Rabbit pAb(bs-7731R)-50ul/100ul/200ul
￥1180 - 2800
