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TNFAIP3 interacting protein 3

Rabbit pAb(bs-7551R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-7551R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-7551R
    英文名称TNFAIP3 interacting protein 3 Rabbit pAb
    中文名称肿瘤坏死因子α诱导相互作用蛋白3抗体
    英文别名Listeria induced; A20-binding inhibitor of NF-kappa-B activation 3; ABIN-3; ABIN-3 beta; FLJ21162; LIND; Listeria induced gene protein; TNFAIP3 interacting protein 3; TNFAIP3-interacting protein 3 beta; TNIP3; TNIP3 beta; TNIP3_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human, Cow, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human TNFAIP3 interacting protein 3/ABIN3
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量39 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators

    Cardiovascular > Vasculature > Endothelium

    Signal Transduction > Signaling Pathway > Nuclear Signaling > NFkB Pathway

    亚基Interacts with TNFAIP3. Interacts with polyubiquitin.
    组织特异性Highly expressed in lung, lymph node, thymus and fetal liver. Expressed at lower levels in bone marrow, brain, kidney, spleen, leukocytes and tonsils. Could be detected in heart, salivary gland, adrenal gland, pancreas, ovary and fetal brain. High levels detected in liver, colon, small intestine, muscle, stomach, testis, placenta, thyroid, uterus, prostate, skin and PBL.
    功能Binds to zinc finger protein TNFAIP3 and inhibits NF-kappa-B activation induced by tumor necrosis factor, Toll-like receptor 4 (TLR4), interleukin-1 and 12-O-tetradecanoylphorbol-13-acetate. Overexpression inhibits NF-kappa-B-dependent gene expression in response to lipopolysaccharide at a level downstream of TRAF6 and upstream of IKBKB. NF-kappa-B inhibition is independent of TNFAIP3 binding.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料ABIN-3 is a member of the A20-binding inhibitor of NF-kappaB activation (ABIN) protein family. Similar to the previously characterized human ABINs (ABIN-1 and ABIN-2), ABIN-3 can bind to A20 and inhibit NF-kappaB activation. In contrast, mouse ABIN-3 is incapable of inhibiting NF-kappaB activation by proinflammatory stimuli because the protein lacks a complete ABIN homology domain, which is required for the funcitonal activity of human ABIN-3.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Sample:
    Lane 1: Mouse Cerebrum tissue lysates
    Lane 2: Mouse Adrenal gland tissue lysates
    Lane 3: Mouse Kidney tissue lysates
    Lane 4: Rat Cerebrum tissue lysates
    Lane 5: Rat Spleen tissue lysates
    Lane 6: Rat Adrenal gland tissue lysates
    Lane 7: Rat Kidney tissue lysates
    Primary: Anti-TNFAIP3 interacting protein 3 (bs-7551R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 39 kDa
    Observed band size: 40 kDa
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TNFAIP3 interacting protein 3) Polyclonal Antibody, Unconjugated (bs-7551R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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