Blank control (black line): A431(black) (The cells were fixed with 2% Paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-Phospho-Desmin(Thr76+Thr77) antibody (bs-5302R) ;
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
- ￥1180 - 2800
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- bs-5302R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5302R |
| 英文名称 | phospho-Desmin (Thr76 + Thr77) Rabbit pAb |
| 中文名称 | 磷酸化结蛋白抗体 |
| 英文别名 | Desmin(phospho T76/77); Desmin(phospho Thr76/Thr77); Desmin(phospho Thr76+Thr77); DESM_HUMAN; DES; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, colon, skeletal muscle, skeletal muscle, skeletal muscle, skeletal muscle, heart, heart, heart, heart |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human DES around the phosphorylation site of Thr76/Thr77 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Extracellular matrix Cardiovascular > Heart > Cardiac arrhythmias Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class III > Desmin Stem Cells > Mesenchymal Stem Cells > Myogenesis |
| 亚基 | Homopolymer. Interacts with DST. Interacts with MTM1. |
| 亚细胞定位 | Cytoplasm. |
| 翻译后修饰 | ADP-ribosylation prevents ability to form intermediate filaments. |
| 相似性 | Belongs to the intermediate filament family. |
| 功能 | Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Desmin is a muscle-specific, type III intermediate filament that integrates the sarcolemma, Z disk, and nuclear membrane in sarcomeres and regulates sarcomere architecture. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z line structures. Defects in Desmin are the cause of desmin related cardio skeletal myopathy (CSM) also known as desmin related myopathy (DRM). CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by intracytoplasmic accumulation of desmin reactive deposits in cardiac and skeletal muscle cells. A desmin related myopathy can have a distal onset, it is then known as hereditary distal myopathy (HDM). Defects in Desmin are also the cause of dilated cardiomyopathy type 1I (CMD1I). CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation and impaired systolic function. Antidesmin antibodies are useful in identification of tumours of myogenic origin |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Desmin (Thr76 + Thr77)) Polyclonal Antibody, Unconjugated (bs-5302R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Desmin (Thr76 + Thr77)) Polyclonal Antibody, Unconjugated (bs-5302R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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phospho-Desmin (Thr76 + Thr77) Rabbit pAb(bs-5302R)-50ul/100ul/200ul
￥1180 - 2800
