Tissue/cell: human skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCDC68 Polyclonal Antibody, Unconjugated(bs-8086R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-8086R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8086R |
| 英文名称 | CCDC68 Rabbit pAb |
| 中文名称 | 卷曲螺旋结构域蛋白68抗体 |
| 英文别名 | CCD68_HUMAN; se57-1; CCDC 68; CCDC68; Coiled coil domain containing 68; Coiled coil domain containing protein 68; Coiled-coil domain-containing protein 68; CTCL associated antigen se57 1; CTCL tumor antigen se57 1; CTCL-associated antigen se57-1; Cutaneous T cell lymphoma associated antigen; Cutaneous T cell lymphoma associated antigen se57 1; Cutaneous T-cell lymphoma-associated antigen se57-1; FLJ25368; SE57 1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from Human CCDC68/se57-1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies |
| 组织特异性 | Expressed in bone marrow, colon, small intestine, spleen, testis, trachea and cutaneous T-cell lymphoma (CTCL). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CCDC68, also known as CTCL tumor antigen se57-1, is a 335 amino acid protein expressed in cutaneous T-cell lymphoma (CTCL), bone marrow, colon, small intestine, spleen, testis and trachea tissues. CTCL is a cancer of the immune system associated with mutation of the T cells. Malignant T cells form lesions on the surface of the skin. se57-1 contains 1 coiled coil domain and is encoded by the CCDC68 gene mapping to human chromosome 18. Chromosome 18 houses over 300 protein-coding genes and contains nearly 76 million bases. There are a variety of diseases associated with defects in chromosome 18-localized genes, some of which include Trisomy 18 (also known as Edwards syndrome), Niemann-Pick disease, hereditary hemorrhagic telangiectasia, erythropoietic protoporphyria and follicular lymphomas. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCDC68 Polyclonal Antibody, Unconjugated(bs-8086R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCDC68 Polyclonal Antibody, Unconjugated(bs-8086R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample: Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-CCDC68 (bs-8086R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Primary: Anti-CCDC68 (bs-8086R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
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CCDC68 Rabbit pAb(bs-8086R)-50ul/100ul/200ul
￥1180 - 2800
