SMC2 Rabbit pAb(bs-7727R)-50ul/100ul/200ul

SMC2 Rabbit pAb(bs-7727R)-50ul

/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-7727R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-7727R
    英文名称SMC2 Rabbit pAb
    中文名称染色体结构维持蛋白2抗体
    英文别名CAP E; CAPE; Chromosome associated protein E; Chromosome-associated protein E; FLJ10093; hCAP E; hCAP-E; hCAPE; PRO0324; SMC 2; SMC protein 2; SMC-2; SMC2; SMC2 L1; SMC2_HUMAN; SMC2L1; Structural maintenance of chromosomes(SMC) family member chromosome associated protein E; Structural maintenance of chromosomes 2; Structural maintenance of chromosomes 2 like 1; Structural maintenance of chromosomes 2 yeast like 1; Structural maintenance of chromosomes protein 2; XCAP E homolog; XCAP-E homolog; XCAPE homolog.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human SMC2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量136 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cell Biology > Cell Cycle > Cell Division > Chromatid Cohesion

    Epigenetics and Nuclear Signaling > Cell cycle > Chromosome Structure > Chromatid Cohesion

    Epigenetics and Nuclear Signaling > Chromosome Structure > Cohesins

    亚基Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG.
    亚细胞定位Nucleus. Cytoplasm. Chromosome. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
    相似性Belongs to the SMC family. SMC2 subfamily.
    功能Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    SMC2 Rabbit pAb(bs-7727R)-50ul
    Sample:
    Jurkat(Human) Cell Lysate at 30 ug
    Primary: Anti- SMC2 (bs-7727R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 136 kD
    Observed band size: 136 kD
    SMC2 Rabbit pAb(bs-7727R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    SMC2 Rabbit pAb(bs-7727R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    SMC2 Rabbit pAb(bs-7727R)-50ul
    Sample:
    MOLT-4(Human) Cell Lysate at 30 ug
    Siha(Human) Cell Lysate at 30 ug
    Primary: Anti-SMC2 (bs-7727R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 136 kD
    Observed band size: 136 kD

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