Sample: Lymph node(Mouse)Lysate at 40 ug
_x000D_
Primary: Anti-p-MEF2C(Ser396)(bs-5480R)at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
_x000D_
Predicted band size: 51kD
_x000D_
Observed band size: 51kD
- ￥1180 - 2800
- Bioss
- bs-5480R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5480R |
| 英文名称 | phospho-MEF2C (Ser396) Rabbit pAb |
| 中文名称 | 磷酸化肌细胞增强因子2C抗体 |
| 英文别名 | MEF2C(phospho S396); p-MEF2C(phospho S396); MEF2C(phospho S396); MADS box transcription enhancer factor 2 polypeptide C(myocyte enhancer factor 2C); Myocyte enhancer factor 2C; Myocyte specific enhancer factor 2C; Similar to MADS box transcription enhancer factor 2 polypeptide C; MEF2C_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human MEF2C around the phosphorylation site of Ser396 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiogenesis > Transcription factors/regulators Cardiovascular > Heart > Hypertrophy > Transcription factors Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Transcription > Other factors Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Neuroscience > Neurology process > Neurogenesis Signal Transduction > Signaling Pathway > Calcium Signaling > Calmodulin Pathway Stem Cells > Mesenchymal Stem Cells > Myogenesis |
| 亚基 | Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation. Interacts with EP300 in differentiating cells; the interaction acetylates MEF2C leading to increased DNA binding and activation. Interacts with HDAC7 and CARM1 (By similarity). Interacts with HDAC4, HDAC7 AND HDAC9; the interaction WITH HDACs represses transcriptional activity. Interacts with MYOCD (By similarity). |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in brain and skeletal muscle. |
| 翻译后修饰 | Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation. Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity. Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. |
| 相似性 | Belongs to the MEF2 family. Contains 1 MADS-box domain. Contains 1 Mef2-type DNA-binding domain. |
| 功能 | Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
_x000D_ Lane 1: Mouse Cerebrum tissue lysates
_x000D_ Lane 2: Mouse Muscle tissue lysates
_x000D_ Lane 3: Rat Cerebrum tissue lysates
_x000D_ Lane 4: Rat Muscle tissue lysates
_x000D_ Lane 5: Rat Spleen tissue lysates
_x000D_ Primary: Anti-phospho-MEF2C (Ser396) (bs-5480R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 51 kDa
_x000D_ Observed band size: 51 kDa
_x000D_ Lane 1: Mouse Cerebrum tissue lysates
_x000D_ Lane 2: Mouse Muscle tissue lysates
_x000D_ Lane 3: Rat Cerebrum tissue lysates
_x000D_ Lane 4: Rat Muscle tissue lysates
_x000D_ Lane 5: Rat Spleen tissue lysates
_x000D_ Primary: Anti-phospho-MEF2C (Ser396) (bs-5480R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 51 kDa
_x000D_ Observed band size: 51 kDa
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[IF=3.913] Fengyun Wen. et al. The Mef2c/AdipoR1 axis is responsible for myogenic differentiation and is regulated by resistin in skeletal muscles. GENE. 2023 Mar;857:147193 WB Mouse
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MEF2C (p-S396) Rabbit pAb(bs-5480R)-50ul/100ul/200ul
￥1180 - 2800
