Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MAP4 (Ser1046) (bs-5439R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 117 kD
- ￥1180 - 2800
- Bioss
- bs-5439R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5439R |
| 英文名称 | phospho-MAP4 (Ser1046) Rabbit pAb |
| 中文名称 | 磷酸化微管相关蛋白4抗体 |
| 英文别名 | MAP4(phospho Ser1046); MAP4(phospho S1046); DKFZp779A1753; MAP-4; MAP4; MAP4_MOUSE; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from mouse MAP4 around the phosphorylation site of Ser1046 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 117 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cyclins > Cyclin B Family Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microtubules > MT Associated Proteins > MAP |
| 亚基 | Interacts with SEPT2; this interaction impedes tubulin-binding (By similarity). |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 组织特异性 | Testis, striated and cardiac muscle. |
| 翻译后修饰 | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-760 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 4 is phosphorylated on Ser-333 and Ser-334 (By similarity). |
| 相似性 | Contains 4 Tau/MAP repeats. |
| 功能 | Non-neuronal microtubule-associated protein. Promotes microtubule assembly. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the C-terminus negatively regulates MAP4 activity to promote microtubule assembly. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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phospho-MAP4 (Ser1046) Rabbit pAb(bs-5439R)-50ul/100ul/200ul
￥1180 - 2800
