Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
- ￥1180 - 2800
- Bioss
- bs-3900R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3900R |
| 英文名称 | CYP2R1 Rabbit pAb |
| 中文名称 | 细胞色素P450 2R1抗体 |
| 英文别名 | Cytochrome P450 2R1; Cytochrome P450 family 2 R1; Cytochrome P450 family 2 subfamily R polypeptide 1; EC 1.14.13.15; MGC4663; Vitamin D 25 hydroxylase. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CYP2R1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cytochromes Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Metabolism > Pathways and Processes > Metabolic signaling pathways > Drug metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipases Metabolism > Pathways and Processes > Mitochondrial Metabolism > Cytochromes Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Signal Transduction > Metabolism > Drug metabolism |
| 亚基 | Homodimer. |
| 亚细胞定位 | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| 组织特异性 | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| 相似性 | Belongs to the cytochrome P450 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor.Defects in CYP2R1 are a cause of 25-hydroxyvitamin D(3) deficiency, also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroxyvitamin D. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
Sample:
Liver(Human) Cell Lysate at 40 ug
Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Liver(Human) Cell Lysate at 40 ug
Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
A431(Human) Cell Lysate at 30 ug
Primary: Anti- CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Blank control:Jurkat.
Primary Antibody (green line): Rabbit Anti-CYP2R1 antibody (bs-3900R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CYP2R1 antibody (bs-3900R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 8.2 }}] {Qingjing Gao. et al. 1,25(OH)₂D₃ regulates androgen synthesis via transcriptional control of steroidogenic enzymes and LHR in the scented glands of muskrats (Ondatra zibethicus). FREE RADICAL BIO MED. 2025 Mar;229:82} {IHC} {Muskrat}
[IF={{ 4.8 }}] {Wenjing Lu. et al. Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells. BBA-MOL CELL BIOL L. 2024 May;1869:159469} {IHC} {Muskrat}
[IF={{ 4.3 }}] {Xu Jia-Yi. et al. Long noncoding RNA XLOC_006786 inhibits the proliferation, invasion and metastasis of osteosarcoma cells through NOTCH3 signaling pathway by targeting miR-491-5p. HUM CELL. 2023 Aug;:1-12} {Other} {}
[IF={{ 4.285 }}] {Stenhouse Claire. et al. Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling. Biol Reprod. 2022 Feb;:} {IHC} {Sheep（Ewe）}
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CYP2R1 Rabbit pAb(bs-3900R)-50ul/100ul/200ul
￥1180 - 2800
