Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-PLASTIN3 (bs-3643R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
- ￥1180 - 2800
- Bioss
- bs-3643R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 详细信息
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3643R |
| 英文名称 | PLASTIN3 Rabbit pAb |
| 中文名称 | 丝束蛋白T Plastin抗体 |
| 英文别名 | Plastin 3(T isoform); PLS3; PLS 3; PLS-3; Plastin 3; Plastin3; Plastin-3; T fimbrin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PLASTIN3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 69 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Crosslinking Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Focal Adhesions |
| 亚基 | Monomer. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Expressed in a variety of organs, including muscle, brain, uterus and esophagus. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR |
| 相似性 | Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains. |
| 功能 | Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | T Plastin is a representative of an important family of actin-cross-linking and -bundling proteins that organize most of the actin networks in cells. T Plastin is associated with the actin core bundle of microvilli, stereocilia and microspikes in epithelial cells and was proposed to participate in the assembly of these structures. In mesenchymal cells, T plastin localizes to focal adhesions and the leading edge, a broad lamellar membrane extension harboring a dynamic branched actin network. Recent studies have shown that T plastin has the potential to be a Sezary cell-specific marker valuable for diagnostic and treatment of Sezary syndrome. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLASTIN3) Polyclonal Antibody, Unconjugated (bs-3643R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PLASTIN3 antibody (bs-3643R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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PLASTIN3 Rabbit pAb(bs-3643R)-50ul/100ul/200ul
￥1180 - 2800
