Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ALPPL2 Polyclonal Antibody, Unconjugated(bs-20188R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-20188R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20188R |
| 英文名称 | ALPPL2 Rabbit pAb |
| 中文名称 | 碱性磷酸酶(类胎盘型)抗体 |
| 英文别名 | Akp5; Alkaline phosphatase; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase placental like; ALP 1; ALP-1; ALP1; ALPG; ALPPL 2; ALPPL; C77216; D1Ertd816e; GCAP; Germ cell alkaline phosphatase; Nagao isozyme; Placental alkaline phosphatase-like; Placental like alkaline phosphatase; placental-like; PLAP like; PLAP-like; PPBN_HUMAN; Testicular and thymus alkaline phosphatase. |
| 中文别名 | 类胎盘碱性磷酸酶; 碱性磷酸酶类胎盘; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ALPPL2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 53 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > Alkaline Phosphatase |
| 亚基 | Homodimer. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 组织特异性 | Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors. |
| 相似性 | Belongs to the alkaline phosphatase family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line):Rabbit Anti-ALPPL2 antibody (bs-20188R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Primary Antibody (green line):Rabbit Anti-ALPPL2 antibody (bs-20188R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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文献和实验该产品被引用文献
[IF={{ 10.383 }}] {Peiyang Gu. et al. Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXX} {WB} {Mouse}
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ALPPL2 Rabbit pAb(bs-20188R)-50ul/100ul/200ul
￥1180 - 2800
