Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GSK3 Alpha(Ser21) Polyclonal Antibody, Unconjugated(bs-4692R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-4692R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4692R |
| 英文名称 | phospho-GSK3A (Ser21) Rabbit pAb |
| 中文名称 | 磷酸化糖原合酶激酶-3α抗体 |
| 英文别名 | GSK3A_HUMAN; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase 3 alpha; EC:2.7.11.26; Serine/threonine-protein kinase GSK3A; EC:2.7.11.1; GSK 3 alpha; GSK3alpha; GSK-3 alpha; GSK3 alpha; GSK-3alpha; GSK 3A; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human GSK3A around the phosphorylation site of Ser21 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 54 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Heart > Apoptosis Cardiovascular > Heart > Hypertrophy Cell Biology > Cell Cycle > Kinases/Phosphatases > Cdks Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases Stem Cells > Signaling Pathways > Wnt > Cytoplasmic |
| 亚基 | Monomer. Interacts with ARRB2. Interacts with AXIN1 and CTNNB1/beta-catenin. |
| 翻译后修饰 | Phosphorylated by AKT1 at Ser-21: upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3A, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-279. |
| 相似性 | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain. |
| 功能 | Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transctiption factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Mouse Cerebrum tissue lysates
Primary: Anti-phospho-GSK3A (Ser21) (bs-4692R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 53 kDa
Lane 1: Mouse Thymus tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Mouse Cerebrum tissue lysates
Primary: Anti-phospho-GSK3A (Ser21) (bs-4692R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 53 kDa
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[IF={{ 4.2 }}] {Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).} {IHC} {="Mouse"}
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phospho-GSK3A (Ser21) Rabbit pAb(bs-4692R)-50ul/100ul/200ul
￥1180 - 2800
