Blank control: K562.
Primary Antibody (green line): Rabbit Anti-phospho-MyoD1 (Ser200) antibody (bs-5499R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-5499R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
ACSBG2 Rabbit pAb(bs-8008R)-50ul/100ul/200ul
￥1180phospho-c-Jun (Ser63) Recombinant Rabbit mAb(bsm-52141R)-50ul/100ul
￥1400
phospho-HER2 (Tyr1221) Rabbit pAb, BF405 conjugated(bs-13090R-BF405)-100ul
￥2980
NMUR2 Rabbit pAb(bs-11421R)-50ul/100ul/200ul
￥1180
HSD17B1 Recombinant Rabbit mAb(bsm-60664R)-50ul/100ul/25ul
￥800
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5499R |
| 英文名称 | phospho-MyoD1 (Ser200) Rabbit pAb |
| 中文名称 | 磷酸化肌原调节蛋白抗体 |
| 英文别名 | MyoD1(phospho Ser200); MyoD1(phospho S200); p-MyoD1(phospho S200); MYOD1(phospho S200); Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human MyoD1 around the phosphorylation site of Ser200 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 34 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Organogenesis > Skeletal development > Muscle Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH |
| 亚基 | Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5 (By similarity). |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). Ubiquitinated on the N-terminus; which is required for proteasomal degradation. |
| 相似性 | Contains 1 bHLH (basic helix-loop-helix) domain. |
| 功能 | Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Tongue tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Primary: Anti- phospho-MyoD1 (Ser200) (bs-5499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 47 kDa
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Tongue tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Primary: Anti- phospho-MyoD1 (Ser200) (bs-5499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 47 kDa
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 4.938 }}] {Du J et al. The regulation of skeletal muscle fiber-type composition by betaine is associated with NFATc1/MyoD.J Mol Med (Berl). 2018 Jun 6.} {WB} {Mouse}
技术资料暂无技术资料 索取技术资料
文献支持
phospho-MyoD1 (Ser200) Rabbit pAb(bs-5499R)-50ul/100ul/200ul
￥1180 - 2800
