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phospho-MAP3K7IP1 (Thr431) Rab

bit pAb(bs-5496R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-5496R
  • 2025年10月24日
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-5496R
    英文名称phospho-MAP3K7IP1 (Thr431) Rabbit pAb
    中文名称磷酸化转化生长因子β活化激酶结合蛋白1抗体
    英文别名MAP3K7IP1(phospho S431); MAP3K7IP1(phospho S429) mo, rat; MAP3K7IP 1; MAP3K7IP1; Mitogen activated protein kinase kinase kinase 7 interacting protein 1; TAB 1; TAK1 binding protein 1; TGF beta activated kinase 1 binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; Transforming growth factor beta activated kinase binding protein 1; 2310012M03Rik; 3'-Tab1; MGC57664; TAB1_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human MAP3K7IP1 around the phosphorylation site of Thr431
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量56 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators

    Immunology > Innate Immunity > TLR Signaling

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway

    亚基Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2.
    组织特异性Ubiquitous.
    翻译后修饰Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.
    相似性Contains 1 PP2C-like domain.
    功能May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta and members of the Toll IL 1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals. It has been shown that the C terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N terminus acts as a dominant negative inhibitor of TGF beta, demonstrating how this protein can function as a mediator between TGF beta receptors and TAK1.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Sample: 293T Cell (Human) Lysate at 40 ug
    Primary: Anti-phospho-MAP3K7IP1 (Thr431)(bs-5496R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 56kD
    Observed band size: 60kD
    产品细节图片2
    Sample:
    U937 Cell (Human) Lysate at 40 ug
    Hela Cell (Human) Lysate at 40 ug
    Siha Cell (Human) Lysate at 40 ug
    Primary: Anti-phospho-MAP3K7IP1 (Thr431)(bs-5496R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 56kD
    Observed band size: 65kD
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAP3K7IP1 (Thr431)) Polyclonal Antibody, Unconjugated (bs-5496R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    ￥1180 - 2800