Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃).
Primary Antibody (green line): Rabbit Anti-LC3A/B antibody (bs-20414R), Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
- ￥1180 - 2800
- Bioss
- bs-20414R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20414R |
| 英文名称 | LC3A/B Rabbit pAb |
| 中文名称 | 自噬微管相关蛋白轻链3A/3B抗体 |
| 英文别名 | MLP3A_HUMAN; Microtubule-associated proteins 1A/1B light chain 3A; MAP1LC3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A(MAP1A/MAP1B LC3 A); Microtubule-associated protein 1 light chain 3 alpha; MLP3B_HUMAN; Microtubule-associated proteins 1A/1B light chain 3B; MAP1LC3B; MAP1ALC3; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B(MAP1A/MAP1B LC3 B); Microtubule-associated protein 1 light chain 3 beta; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Cow, Horse, Sheep, GuineaPig, Goat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LC3A/B |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 14 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究领域 | Cancer > Cell Death > Autophagy > APG gene products Cancer > Cell Death > Autophagy > Signal Transduction Cancer > Signal transduction > Autophagy Cardiovascular > Heart > Autophagy > APG gene products Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy > APG gene products Neuroscience > Neurology process > Neurogenesis Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microtubules > MT Associated Proteins > MAP |
| 亚基 | 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with SQSTM1. Interacts with TP53INP1 and TP53INP2. |
| 亚细胞定位 | Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes. |
| 组织特异性 | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| 翻译后修饰 | The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. |
| 相似性 | Belongs to the MAP1 LC3 family. |
| 功能 | Probably involved in formation of autophagosomal vacuoles (autophagosomes). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A/B) Polyclonal Antibody, Unconjugated (bs-20414R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 7.086 }}] {Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625} {IF} {Mouse}
[IF={{ 6.331 }}] {Maowen Chen. et al. Enhanced biocompatibility and osteogenic differentiation of mesenchymal stem cells of titanium by Sr–Ga clavate double hydroxides. J Mater Chem B. 2021 Jul;:} {IF} {Rat}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Aging：新型假马齿苋皂苷通过免疫调节方式清除淀粉样蛋白，改善 APP/PS1 小鼠的认知损伤
具有抗脑缺血、抗精神抑郁症、抗精神分裂症、抗焦虑和抗 AD 的作用，但是有效成分不清，作用机制不明。 张卫东教授课题组长期致力于基于中药的新药发现。课题组前期研究发现假马齿苋皂苷 Bacopaside I（BS-I）具有很好的保护神经细胞、治疗脑缺血和抑郁症的作用。本项研究进一步发现 BS-I 能显著减少 APP/PS1 转基因小鼠 b 淀粉样蛋白斑块负荷、改善其学习能力和长期空间记忆障碍。综合利用基因组学和蛋白质组学技术，发现 BS-I 通过调节先天免疫和吞噬作用，促进淀粉
.9| Mus musculus chromosome 16, clon... 44 0.13 gi|13435314|gb|AC090999.1| Caenorhabditis elegans cosmid Y8... 44 0.13 gi|21955645|emb|AL732599.5| Mouse DNA sequence from clone R... 44 0.13 gi|199921|gb|J03368.1|MUSMX2 Mouse interferon-induced Mx2 m
技术资料暂无技术资料 索取技术资料
文献支持
LC3A/B Rabbit pAb(bs-20414R)-50ul/100ul/200ul
￥1180 - 2800
