Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Angiotensin III antibody (bs-20101R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
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- bs-20101R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20101R |
| 英文名称 | Angiotensin III Rabbit pAb |
| 中文名称 | 血管紧张素III抗体 |
| 英文别名 | ANGT_HUMAN; Angiotensin-1; Angiotensin I; Ang I; Angiotensin-2; Angiotensin II; Ang II; Ang III; Angiotensin-3; Angiotensin III; Angiotensin3; Angiotensin 3; Angiotensin-4; Angiotensin 1-4; Angiotensin 1-5; Angiotensin 1-7; Angiotensin 1-8; Angiotensin 2-8; Angiotensin 3-8; Angiotensin 1-9; Angiotensin 1-10; Angiotensinogen; Serpin A8; Angiotensin IV; Ang IV; ANHU; Des-Asp[1]-angiotensin II; SERPINA8. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide of human Angiotensin III (RVYIHPF) |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 1/50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cardiovascular > Blood > Serum Proteins Metabolism > Pathways and Processes > Metabolism processes > Hypoxia |
| 亚基 | During pregnancy, exists as a disulfide-linked 2:2 heterotetramer with the proform of PRG2 and as a complex (probably a 2:2:2 heterohexamer) with pro-PRG2 and C3dg. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed by the liver and secreted in plasma. |
| 翻译后修饰 | Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin. |
| 相似性 | Belongs to the serpin family. |
| 功能 | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3: stimulates aldosterone release. Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-0587P is a Eight branched multiple antigenic peptide of Angiotensin II. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Plasma (Mouse) at 20 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Adrenal gland (Mouse) Lysate at 40 ug
Lane 5: Adrenal gland (Rat) Lysate at 40 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Angiotensin III (bs-20101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52/60 kD
Observed band size: 52/60 kD
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Plasma (Mouse) at 20 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Adrenal gland (Mouse) Lysate at 40 ug
Lane 5: Adrenal gland (Rat) Lysate at 40 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Angiotensin III (bs-20101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52/60 kD
Observed band size: 52/60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin III) Polyclonal Antibody, Unconjugated (bs-20101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin III) Polyclonal Antibody, Unconjugated (bs-20101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 3.367 }}] {Hua Chen. et al. Chronic Intermittent Hypobaric Hypoxia Decreases High Blood Pressure by Stabilizing the Vascular Renin-Angiotensin System in Spontaneously Hypertensive Rats. Front Physiol. 2021; 12: 639454} {IHC} {Rat}
[IF={{ 3.2 }}] {Delong Duo. et al. Long-term exposure to high-altitude hypoxic environments reduces blood pressure by inhibiting the renin-angiotensin system in rats. FRONT PHYSIOL. 2025 Apr;16:1565147} {WB} {Rat}
相关实验
Characterization of Angiotensin II Receptors
. Chang, R.S.L., Lotti, V.J., Chen, T.B., and Faust, K.A. 1990. Two angiotensin II binding sites in rat brain revealed using 125I‐Sar1‐Ile8‐angiotensin II and selective nonpeptide antagonists. Biochem. Biophys
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
and angiotensin II‐induced abdominal aortic aneurysm formation. Arterioscler. Thromb. Vasc. Biol. 29:1458‐1464. Pyo, R., Lee, J.K., Shipley, J.M., Curci, J.A., Mao, D., Ziporin, S.J
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
human leukemia inhibitory factorS, 100 mg/L, α-MF[179]Rabbit plasma cholesteryl ester transfer proteinS, PHO1[180]Rabbit testicular angiotensin converting enzymeS, PHO1, native[181]Rabbit tissue factor (thromboplastin)I, 92 mg/g wet cell paste, active519
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Angiotensin III Rabbit pAb(bs-20101R)-50ul/100ul/200ul
￥1180 - 2800
