Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PLCZ1 Polyclonal Antibody, Unconjugated(bs-5378R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-5378R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5378R |
| 英文名称 | PLCZ1 Rabbit pAb |
| 中文名称 | 磷酸肌醇磷脂酶PLCZ1抗体 |
| 英文别名 | 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase zeta 1; phosphoinositide phospholipase C zeta 1; phospholipase C zeta 1; PI phospholipase C zeta 1; PLC zeta 1; PLCzeta; Testis development protein NYD SP27; Testis development related NYD SP27; MGC149685 antibody NYD SP27 antibody. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PLCZ1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 70 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism |
| 组织特异性 | Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, PLCZ1 hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. It triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. PLCZ1 is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. It may exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (bs-5378R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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文献和实验该产品被引用文献
[IF={{ 6.684 }}] {Meng Wang. et al. A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure. Front Cell Dev Biol. 2021; 9: 672081} {IF} {Human}
[IF={{ 4.5 }}] {Meng Wang. et al. Novel ACTL7A variants in males lead to fertilization failure and male infertility. ANDROLOGY-US. 2023 Nov;:} {IF} {Human}
[IF={{ 3.322 }}] {Xiaopei Zhou. et al. Actl7a deficiency in mice leads to male infertility and fertilization failure. BIOCHEM BIOPH RES CO. 2022 Oct;623:154} {IF} {Mouse}
[IF={{ 3.2 }}] {Rui Guo. et al. Homozygous deleterious variants in the C-terminal of TDRD5 impair spermiogenesis, causing severe oligoasthenoteratozoospermia in humans. ANDROLOGY-US. 2024 Jun;:} {IF} {Human}
[IF={{ 2.98 }}] {Zhou, Xiaopei. et al. A novel homozygous mutation in ACTL7A leads to male infertility. MOL GENET GENOMICS. 2022 Dec;:1-8} {IF} {Human}
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PLCZ1 Rabbit pAb(bs-5378R)-50ul/100ul/200ul
￥1180 - 2800
